Home > Research Groups > Xingzhong Liu > Research Interests and Results > Fungal diversity in special environments

The fungi in special environments are an important component of biodiversity, but most of them in such environments remain unknown. Based on both culture-dependent and culture-independent methods, fungi from wetland, psychrophilic, lithophilic habitants as well as Chinese cordyceps colonizing fungi were investigated.  Two new genera and 48 new species were established

 

 


Fungi in Wetland: Soil samples of 23 typical wetlands from China were collected. The culture-dependent methods resulted in a greater species diversity (183 OTUs), and the most frequent fungi were Ascomycota, including species of Penicillium, Fusarium, Aspergillus, Trichoderma and Talaromyces. PCR-DGGE analysis showed less diversity (145 OTUs). Cluster analysis showed that all samples formed two groups along Qingling-Huaihe Line. The samples from Qinghai-Tibetan were separated from other samples from north of China. Ordination analyses indicated that fungal community structure was strongly correlated with altitude, temperature and total P (p<0.01) (Fig. 1).

 

    Lithobiotic Fungi: Those fungi are apparently lack sexual reproductive structures and form compact, melanised colonies on bare rock surfaces (Fig. 3). Total 303 rocky fungi were isolated and 53 of them were identified which including 19 potential new species. One new species has been described as Rhinocladiella simplex (Fig. 4).

    Chinese Cordyceps Colonizing Fungi: With the culture-dependent method, 572 fungal strains were isolated, and they belonged to 92 putative operational taxonomic units (OTUs). With the culture-independent method, 118 putative OTUs were detected. The most common OTUs detected using both methods belonged to Ascomycota, and potential novel lineages were detected by each of the two methods (Fig.5). By the analysis of their secondary metabolites, many novel compounds were isolated which have important biological activities, such as antimicrobial, anti-tumor, and anti-HIV.