Fermentation of Fungi 

  中文

Caihong DONG (董彩虹)
Professor

born in November 1973 in Hubei, China    
Tel: +86 10 6480 6138
Email: dongch@im.ac.cn

Work experiences

• Professor, 2014, Institute of Microbiology, CAS 

• Associate Professor, 2006, Institute of Microbiology, CAS
• Associate Professor, 2005, Three Gorges University

Education
• Ph.D., 2006, Institute of Microbiology, CAS
• M.S.,1997, Department of Food science, Huazhong Agricultural University
• B.S., 1994, Department of Agronomy, Yangtze University 

Research Interests
• Fermentation of medicinal and edible fungi, Metabolite production and its regulation

Current research

（1）Culture of the medicinal fungus Sparassis crispa
We focused on the Physiological study of this important medicinal fungus Sparassis crispa. The medium and environmental conditions for this fungus has been optimizing now.
（2）Cloning and analysis of the Oswc-1 gene encoding a putative blue light photoreceptor from Ophiocordyceps sinensis
Light plays an important role during both sexual and asexual growth of Ophiocordyceps sinensis, one of the best known traditional Chinese medicines. In the present study, we cloned Oswc-1, the homologue of the blue-light photoreceptor Ncwc-1 of Neurospora crassa, from O. sinensis by Hi-tail PCR. The deduced amino acid sequence of Oswc-1 contains the similar function domains as NcWC-1 including transcriptional activation, LOV (Light, Oxygen, or Voltage), PAS (Per-Arnt-Sim) and Zinc Finger domains. Phylogenetic analysis based on fungal WC-1 supported OsWC-1 was a blue light receptor. The expression of Oswc-1 mRNA was up-regulated upon irradiation significantly.
（3）Artificial cultivation of some edible fungi.

Research team

Student： Yang Tao  , Lian Tiantian

Representative Publications

Sun JZ, Dong CH*, Liu XZ, Liu JK & Hyde KD. 2016. Calcarisporium cordycipiticola sp. nov., an important fungal pathogen of Cordyceps militaris. Phytotaxa 268 (2): 135–144.

Guo MM, Guo SP, Yang HJ, Bu N & Dong CH*. 2016. Comparison of major bioactive compounds of the caterpillar medicinal mushroom, Cordyceps militaris (ascomycetes), fruiting bodies cultured on wheat substrate and pupae. International Journal of Medicinal Mushrooms, 18(4): 327–336

Li S.F., Dong C.H., Wen H.A., and Liu X.Z. 2016. Development of Ling-zhi industry in China- emanated from the artificial cultivation in the Institute of Microbiology, Chinese Academy of Sciences (IMCAS), Mycology: An International Journal on Fungal Biology, DOI: 10.10 80/21501203.2016.1171805

Lu Y.Z., Xia Y.L., Luo F.F., Dong C.H., and Wang C.S.*. 2016. Functional convergence and divergence of mating-type genes fulfilling in Cordyceps militaris, Fungal genetics and biology 88: 35-43.

Yang T., Guo M.M., Yang H.J., Guo S.P. and Dong C.H.* 2016. The blue light receptor CmWC-1 mediates fruit body development and secondary metabolism in Cordyceps militaris. Applied Microbiology and Biotechnology 100: 743-755.

Dong C.H., Guo S.P., Wang W.F. and Liu X.Z. 2015. Cordyceps industry in China. Mycology: An International Journal on Fungal Biology 6(2): 121–129.

Lian T.T, Yang T., Liu G.J., Sun J. D. and Dong C.H. * 2014. Reliable reference gene selection for Cordyceps militaris gene expression studies under different developmental stages and media. FEMS Microbiology Letters 356(1): 97-104.

Lian T.T, Yang T, Sun J.D., Guo S.P., Yang H.J. and Dong C.H. * 2014. Variations of SSU rDNA group I Introns in different isolates of Cordyceps militaris and the loss of an intron during cross-Mating. Journal of Microbiology 52(8): 659-66.

Yang T. and Dong C.H.* 2014. Photo morphogenesis and Photo response of the blue-light receptor gene Cmwc-1 in different strains of Cordyceps militaris. FEMS Microbiology Letters 352(2): 190-197.

Yang T., Xiong W.P. and Dong C.H.* 2014. Cloning and analysis of the Oswc-1 gene encoding a putative blue light photoreceptor from Ophiocordyceps sinensis. Mycoscience 55: 241-245.

Lian T.T., Dong C.H.*, Yang T. and Sun J.D. 2014. Three types of geranylgeranyl diphosphate synthases from the medicinal fungus Cordyceps militaris. International Journal of Medicinal Mushrooms 16(2): 115-124.

Yang T., Sun J.D., Lian T.T., Wang W.Z. and Dong C.H.* 2014. Process optimization for extraction of carotenoids from Cordyceps militaris, an edible and medicinal fungus, International Journal of Medicinal Mushrooms 16(2): 125-135.

Dong C.H.*, Yang T. and Lian T.T. 2014. A comparative study of the antimicrobial, antioxidant and cytotoxic activities of methanol extracts from fruit bodies and fermented mycelia of Cordyceps militaris. International Journal of Medicinal Mushrooms 16(5): 485-95.

Dong C.H. and Yao Y.J.* 2012. Isolation, characterization of melanin derived from Ophiocordyceps sinensis, an entomogenous fungus endemic to the Tibetan Plateau. Journal of Bioscience and Bioengineering 113: 474–479.

Dong C.H. and Yao Y.J.* 2011. On the reliability of fungal materials used in studies on Ophiocordyceps sinensis. Journal of Industrial Microbiology and Biotechnology 38: 1027–1035.

Dong C.H. and Yao Y.J. 2010. Comparison of some metabolites among cultured mycelia of Ophiocordyceps sinensis from different geographical regions. International Journal of Medicinal Mushrooms 12(3): 287–297.

Dong C.H., Xie X.Q., Wang X.L., Zhan Y. and Yao Y.J. 2009. Application of Box-Behnken design in optimisation for polysaccharides extraction from cultured mycelium of Cordyceps sinensis. Food and Bioproducts Processing 87(2): 139–144.

Dong C.H. and Yao Y.J. 2008. In vitro evaluation of antioxidant activities of aqueous extracts from natural and cultured mycelia of Cordyceps sinensis. LWT–Food Science and Technology 41: 699–677.

Yuan W. H., Jiang N., Dong C.H., Wei Z.W., Wu H.K., Chen C.F., Zhao Y.X., Zhou S.L., Zhang M.M., and Zheng W.F. 2013. Lasiodiplodin Analogues from the Endophytic Fungus Sarocladium kiliense. Chem. Pharm. Bull. 61: 363-365.

Zhan Y., Dong C.H. and Yao Y.J. 2006. Antioxidant activities of aqueous extract from cultivated fruit-bodies of Cordyceps militaris in vitro. Journal of Integrative Biology 48: 1365−1370.

Dong C.H. and Yao Y.J. 2005. Nutritional requirements of mycelial growth of Cordyceps sinensis in submerged culture. Journal of Applied Microbiology 99: 483–492.

钱正明, 李春红, 李文庆, 刘铸, 田野, 刘杏忠, 董彩虹*. 2016.冬虫夏草蛋白图谱及干燥条件对超氧化物歧化酶活性影响. 菌物学报35 (4): 424-432.

张晗星，钱正明，苏尧, 刘杏忠，李文佳*，董彩虹*. 2016.冬虫夏草不同发育时期蛋白质组iTRAQ 质谱分析. 菌物学报35 (4): 411-423.

李文佳, 董彩虹*, 刘杏忠, 李全平, 夏金明, 梁蕾. 2016.冬虫夏草培植技术研究进展. 菌物学报35 (4): 375-387.

董彩虹，李文佳，李增智，闫文娟，李泰辉, 刘杏忠*，et al. 2016. 我国虫草产业发展现状、问题及展望----虫草产业发展金湖宣言. 菌物学报35 (1): 1-15.

董彩虹*.2016. 序言II 冬虫夏草：从科学到产业菌物学报35 (4): 369-374.

郭明敏, 杨涛, 卜宁，董彩虹* 2015. 大型真菌光受体及其功能研究进展. 菌物学报34 (5): 880-889.

董彩虹*, 马琪琪. 2014. 珍稀食用菌绣球菌的研究进展. 菌物研究（特约综述）12 (3): 172-177.

廉添添, 杨涛, 孙军德, 黄贤华, 董彩虹*. 2014人工栽培绣球菌的鉴定及其子实体β-葡聚糖含量的酶法测定. 菌物学报33(2): 254-261.

廉添添，董彩虹*，杨涛，孙军德. 2014. 蓝光光照对蛹虫草子实体生长和主要活性成分的影响.菌物学报 33 (4): 838-846.

孙军德，廉添添，杨涛，董彩虹*. 2013. 不同干燥方法对蛹虫草子实体活性成分含量的影响. 沈阳农业大学学报44 (6): 761-765.

何曼妮，杨涛，李晓霞，董彩虹*，杨槐俊. 2013. 从蛹虫草栽培料中分离的单端孢属一中国新记录种. 菌物研究12 (3): 130-132.

杨涛，董彩虹*. 2013. 蛹虫草的光形态建成及蓝光受体基因Cmwc-1. 菌物研究11 (2): 146.

杨涛，董彩虹* . 2011. 虫草素的研究开发现状与思考. 菌物学报 30 (2): 180-190.

马有祥主编，李兵、董彩虹等编撰. 2010. 中国冬虫夏草，新华出版社，ISBN 978-7-5011-9214-4

专利：

董彩虹（2015）一种在蚕蛹上产孢梗束的蝙蝠蛾拟青霉菌株及其培养方法。中国专利申请号：201510512873.5

董彩虹，杨涛（2012）一种广叶绣球菌合成培养基。ZL201210370266.6。2014年3月12日授权。

董彩虹（2011）珍稀食药用菌绣球菌的液体发酵培养方法及其专用培养基。ZL201110220578.4。2013年7月10日授权。

姚一建，董彩虹（2008）一种冬虫夏草发酵菌丝体多糖的提取方法。ZL200810116219.2。2010年12月15日授权。

姚一建，董彩虹（2008）一种从冬虫夏草菌发酵液中提取纯化黑色素的方法。ZL200810241008.1。2012年12月19日授权。

姚一建，董彩虹，谢雪钦，王波（2005）一种冬虫夏草的培养方法及其专用培养基。ZL200510137457.8。2008年5月7日授权。

姚一建，董彩虹（2004）一种冬虫夏草半合成培养基。ZL200410090875.1。2008年3月12日授权。

姚一建，董彩虹（2010）一种蛹虫草固体培养生产类胡萝卜素的方法。中国专利申请号：201010168952.6。