微生物多糖合成途径及功能课题组 组长：金 城 博士 研究员 真菌地衣重点实验室副主任 电话：010-64807425 电子邮件: jinc@sun.im.ac.cn

金城研究员简介

学习经历

1990-1993 中国科技大学北京研究生院博士
1987-1990 复旦大学硕士
1983-1987 复旦大学生物系微生物专业学士

工作简历

2008-至今 中国科学院微生物研究所真菌地衣重点实验室副主任、研究员
1999-2008 中国科学院微生物研究所微生物资源国家重点实验室副主任、研究员
1998-1999 中国科学院微生物研究所微生物资源国家重点实验室副研究员
1997-1998 中国科学院微生物研究所酶学研究室副研究员
1996-1997 美国University of Arkansas for Medical Science生物化学与分子生物学系博士后
1993-1995 中国科学院微生物研究所酶学研究室助理研究员

参加学术团体及任职

2006-至今 中国微生物学会常务理事
2000-至今 国际糖生物学会会员
2001-至今 美国生物化学与分子生物学学会会员
2006-至今 中国微生物学会酶工程专业委员会主任
2003-至今 中国生物工程学会糖生物工程专业委员会主任
2006-至今 中国生物化学与分子生物学学会复合糖专业委员会副主任
2006-至今 中国微生物学会真菌学专业委员会委员
2002-至今 《微生物学通报》副主编

研究方向

    研究方向为重要微生物多糖的生物合成及功能研究。

研究领域

    以重要微生物为模式种，应用功能基因组学、糖生物学、糖链代谢酶的生化与分子生物学手段研究其多糖、糖基化的生物合成途径及其生物学功能。目前开展的研究工作包括：（1）条件致病真菌糖链合成相关的功能基因组学研究，通过分离、鉴定及功能分析来阐明真菌糖蛋白的糖基化修饰在真菌生长和感染过程中的作用；（2）病原细菌胞外多糖的生物合成及功能分析。通过对链球菌、大肠杆菌等病原菌胞外多糖合成相关基因的研究阐明其生物合成途径及胞外多糖对感染的影响；（3）古菌糖蛋白生物合成途径及重要基因/酶的分离。

    近5年来，主持国家自然科学基金课题2项、973子课题2项和863专题1项。已经培养6名博士、5名硕士，正在指导博士研究生10名。1997年以来发表论文30篇，其中在“European Journal of Biochemistry”、“Journal of Biological Chemistry”、“Molecular Microbiology”等SCI收录的刊物上发表论文12篇。

课题组成员

助理研究员

周  慧 博士
欧阳浩淼 硕士

技术员

明  佳 学士

研究生

房文霞 蒋鹤春 吕  洋 阎江洪 李  开 宋丽丽 丁卫平 杜  婷 赵  婉 王婧阳

代表性图片

主要论文

Yang Lü, Haitao Yang, Hongyan Hu, Ying Wang, Zihe Rao, Cheng Jin* (2009). Mutation of Trp137 to glutamate completely removes transglycosyl activity associated with the Aspergillus fumigatus AfChiB1. Glycoconjugate Journal 26:525-534 PDF (IF 1.743)

Wenxia Fang, Xiaoying Yu, Bin  Wang, Hui Zhou, Haomiao Ouyang, Jia Ming and Cheng Jin* (2009). Characterization of the Aspergillus fumigatus phosphomannose isomerase Pmi1 and its impact on cell wall synthesis and morphogenesis. Microbiology-SGM, 155: 3281–3293 PDF (IF 2.841)

Lei Zhang, Deqin Feng, Wenxia Fang, Haomiao Ouyang, Yuanming Luo, Ting Du, and Cheng Jin* (2009), Comparative proteomic analysis of the Aspergillus fumigatus mutant deficient in glucosidase I (AfCwh41). Microbiology-SGM, 155: 2157-2167. PDF (IF 2.841)

Yanjie Li, Wenxia Fang, Lei Zhang, Haomiao Ouyang, Hui Zhou, Yuanming Luo, and Cheng Jin*  (2009), Class IIC α-mannosidase AfAms1 is required for morphogenesis and cellular function in Aspergillus fumigatus. Glycobiology, 19 (6): 624-632. PDF (IF 4.446)

Yanjie Li,Lei Zhang, Depeng Wang, Hui Zhou, Haomiao Ouyang, Jia Ming, and Cheng Jin*. 2008. Deletion of the msdS gene induces abnormal polarity and septation in Aspergillus fumigatus. Microbiology-SGM.154: 1960-1972 PDF (IF 3.110)

Hechun Jiang, Haomiao Ouyang, Hui Zhou & Cheng Jin*. 2008. GDP-mannose pyrophosphorylase is essential for cell wall integrity, morphogenesis and viability of Aspergillus fumigatus. Microbiology-SGM. 154:2730-2739 PDF (IF 3.110)

Lei Zhang, Hui Zhou, Haomiao Ouyang, Yanjie Li, Cheng Jin* 2008. Afcwh41 is required for cell wall synthesis, conidiation, and polarity in Aspergillus fumigatus. FEMS Microbiology  Letters, 289(2): 155-166. PDF (IF 2.274)

Yang Lü, Haitao Yang, Hongyan Hu, Ying Wang, Zihe Rao, Cheng Jin* 2008. Mutation of Trp137 to glutamate completely removes transglycosyl activity associated with the Aspergillus fumigatus AfChiB1. Glycoconjugate Journal (on line) PDF (IF 1.602)

Zhuo, H., Hu, H., Zhang, L., Li, R., Ouyang, H., Ming, J., Jin, C. 2007. Protein O-mannosyltransferase 1 (AfPmt1p) in Aspergillus fumigatus is crucial for cell wall integrity and conidia morphology especially at an elevated temperature. Eukaryotic Cell, 6(12):2260-2268.

Li, H., Zhou, H., Luo, Y., Ouyang, H., Hu, H., Jin, C. 2007. Glycosylphosphatidylinositol (GPI)-anchor is required in Aspergillus fumigatus for morphogenesis and virulence. Mol. Microbiol., 64(4):1014–1027.

Wang, D. P., Li, H. G., Li, Y. J., Guo, S. C., Yang, J., Qi, D. L., Jin, C., and Zhao, X. Q. 2006. Hypoxia-inducible factor 1alpha cDNA cloning and its mRNA and protein tissue specific expression in domestic yak (Bos grunniens) from Qinghai-Tibetan plateau. Biochem. Biophys. Res. Commun., 348(1):310-319.

Yang, X., Yang, S., Han, B., Jin, C. 2005. The structure-function relationship of thermostable -glycosidase from the thermophilic eubacterium Thermus nonproteolyticus HG-102. Chinese J. Biotechnol., 21(1):84-91.

Wang, Y., Wang, J., Hu, H., Yang, X., Yang, S., Yu, X., Jin, C. 2004. Cloning and expression of chitinase from Aspergillus fumigatus YJ-407. Chinese J. Biotechnol., 20(6):843-850.

Liu, G., Jin, C., and Jin, C. 2004. CMP-N-acetylneuraminic acid synthetase from E. coli K1 is a bi-functional enzyme: Identification of minimal catalytic domain for synthetase activity and novel functional domain for platelet-activating factor acetylhydrolase activity. J. Biol. Chem., 279(17): 17738-17749.

Hu, H., Wang, G., Yang, H., Zhou, J., Mo, L., Yang, K., Jin, C., Jin, C., and Rao, Z. 2004. Crystallization and preliminary crystallographic analysis of a native chitinase from the fungal pathogen Aspergillus fumigatus YJ-407. Acta Crystallographica Section D Biological Crystallography, 60(Pt 5):939-940.

Gu, G., Du, Y., Hu, H., Jin, C.2003. Synthesis of 2-chloro-4-nitrophenyl a-L-fucopyranoside: a substrate for a-L-fucosidase (AFU). Carbohydrate Research, 338:1603-1607.

Jin, C. and Jin, C. 2002. Minimal functional domain of cytidine 5’-monophosphate N-acetylneuraminic acid (CMP-NeuAc) synthetase from Escherichia coli. Chinese J. Biotechnol., 18(6):676-682.

Ding, H., Wang, J., Jin, C.? 2002. Regulation of Galb1,3GalNac a2,3-sialyltransferase (ST3Gal I) by hepatitis B virus MHBs^t/HBx transactivator. Chinese J. Biotechnol., 18(5):551-555.

He, X., Jin, C., Zhang, S., Yang, S. 2002. Cloning and expression of a thermostable b-glycosidase gene from Thermus nonproteolyticus HG-102. Chinese J. Biotechnol., 18(1):63.

Xia, G., Jin, C., Zhou, J., Yang, S., Zhang, S., and Jin, C. 2001. A novel chitinase having a unique mode of action from Aspergillus fumigatus YJ-407. Eur. J. Biochem., 268(14):4079-4085.

Liu, J., Zhou, R., Zhang,N., Rui, J., Jin, C. 2000. Biological function of a novel gene overexpressed in human hepatocellular carcinoma. Chinese Med. J., 113(10):881-885.

Xia, G., Han, X., Ding, H., Yang, S., Zhang, S., and Jin, C. 2000. Cloning and overexpression of a cytidine 5’-monophosphate N-acetylneuraminic acid synthetase from Escherichia coli. J. Molecular Catalysis B: Enzymatic, 10:201-208.

Liu, N., Jin, C., Zhu, Z., Tao, H., and Zhang S. 1999. Stage-specific expression of a1,2-fucosyltransferase and a1,3-fucosyltransferase (FT) during mouse embryogenesis. Eur. J. Biochem., 265: 258-263.

Shang, J., Qiu, R., Wang, J., Liu, J., Zhou, R., Yang, S., Zhang S. and Jin, C.1999. Molecular cloning and expression of Gal(1,3GalNAc (2,3-sialyltransferase from human fetal liver. Eur. J. Biochem., 265:580-588.

Shang, J., Qiu, R., Jin, C., Zhang, S. 1999 Cloning of (2,3-sialyltransferase from human fetal liver. Chinese J. Biotechnol., 15(3):277-280.

专利

A novel chitinase produced by Aspergillus fumigatus and its purification. Inventors: Jin, C., Xia, G., Li, Y.-T., Jia, X., Yang, S., Zhang, S. ZL99105415.6，May 19, 2004.

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